What are MCF10A cells?
MCF10A cells are widely used non-malignant breast epithelial cells. They exhibit some features of normal breast epithelium, including lack of anchorage-independent growth and dependence on growth factors and hormones for proliferation and survival [6]. They can form acinar structures in 3D culture [37].
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Is MCF10A cancer cell line?
MCF10A is a spontaneously immortalized, non-malignant breast cell line obtained from a patient with benign fibrocystic disease [32] and is the founder cell line of a progressively more aggressive family of breast cancer lines.
What are NMuMG cells?
NMuMG is a nontransformed mouse mammary gland epithelial cell line that exhibits both a growth inhibitory response and an EMT response to TGF-beta, rendering NMuMG cells a good model system for studying these TGF-beta effects.
What is A549 cell?
A549 cells are adenocarcinomic human alveolar basal epithelial cells, and constitute a cell line that was first developed in 1972 by D. J. Giard, et al. through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58-year-old caucasian male.
What is mcf7 cell line?
1 MCF-7 human adenocarcinoma cells. MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27] …
What is the doubling time for MDA MB 231?
Cell proliferation. The cell counts performed over 72 h of the CD44high/CD24low immunophenotype of the cell lines studied showed that the MDA-MB-231 line had a lower doubling time (41.56 h) than the MDA-MB-436 line (44.79 h) (Figure 5).
What is a triple negative cancer?
The term triple-negative breast cancer refers to the fact that the cancer cells don’t have estrogen or progesterone receptors (ER or PR) and also don’t make any or too much of the protein called HER2. (The cells test “negative” on all 3 tests.)
How many breast cancer cell lines are there?
By analyzing the molecular features of 92 breast cancer cell lines as documented by different literatures, we categorize 84 cell lines into 5 groups to be consistent with breast tumor classification.
Why do we use A549 cells?
The A549 cells have been used to model the alveolar Type II pulmonary epithelium. Studies have shown that this can be particularly useful in research for studying the metabolic processing of lung tissue and for identifying mechanisms of drug delivery to the tissue.
Are A549 cells metastatic?
A549 cells are relatively large cells, with a doubling time of approximately 24 hours. They are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors and metastases following implantation of the cells.
Do MCF-7 cells have HER2?
Two particularly important members of this large family are the epidermal growth factor receptor (EGFR), which is activated by the epidermal growth factor (EGF), and the human epidermal growth factor receptor-2 (HER2), both present in MCF-7 cells (7).
Are MCF-7 cells metastatic?
MCF-7 cells, a human breast carcinoma line, forms tumors when injected into athymic nude mice. These tumors are able to metastasize to lungs, liver and spleen. 17β-estradiol treatment increases both the growth rate and frequency of metastases.
What is the difference between MDA-MB-231 and MDA MB 468?
MDA-MB-157, MDA-MB-231, and BT-549 cells are characterized as triple-negative/basal-B mammary carcinoma, while the MDA-MB-468 cells are characterized as triple-negative/basal-A mammary carcinoma.
What is the difference between MCF-7 and MDA-MB-231?
The MCF-7 cell line has functional estrogen and EGF receptors, is dependent on estrogen and EGF for growth, and is uninvasive, while MDA-MB-231 cells are a model for more aggressive, hormone-independent breast cancer.
Can you survive triple negative cancer?
In general, about 91% of all women with triple-negative breast cancer are still alive 5 years after diagnosis. If the cancer has spread to the lymph nodes near the breast (regional) the 5 year relative survival rate is about 65%. If the cancer has spread to distant places, the 5 year relative survival rate is 12%.
Is triple negative cancer curable?
Survival Rate for Patients with Triple-Negative Breast Cancer. Triple-negative breast cancer is considered aggressive, or fast-growing, but it is treatable. Survival depends on factors such as how advanced the cancer was at diagnosis, your overall health, and your response to treatment.
Why are MCF-7 cells used?
MCF-7 cells are useful for in vitro breast studies because they retained several ideal characteristics particular to mammary epithelium, such as the processing of estrogen, in the form of estradiol, via estrogen receptors (ER) in the cell cytoplasm [28]. It is the first hormone responding breast cancer cell line.
What mutations do A549 cells have?
The A549 cell line is in turn wild-type against other common mutations in lung cancer such as, EGFR, PIK3CA, TP53, ALK and PTEN. The SW900 cell line is also wild-type for these mutations with the exception for the inactivated mutation in the tumor suppressor gene TP53 (homozygous, c.
Why are A549 cells used?
What year were A549 cells established?
The A549 cell line was established in 1972 by D.J. Giard et al, through an explant culture of adenocarcinomic lung tissue of a 58-year-old Caucasian male. A549 cells are hypotriploid alveolar basal epithelial cells.
Does MCF-7 have HER2?
MCF-7 cells do not have amplification of the HER2 (ErbB2) oncogene.
Do MDA-MB-231 cells express HER2?
MDA-MB-231 cells express low levels of HER2.
Is MCF 7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What is MCF7 cell line?
What is the longest survival rate for triple negative BC?
5-year relative survival rates for triple-negative breast cancer
| SEER Stage | 5-year Relative Survival Rate |
|---|---|
| Localized | 91% |
| Regional | 65% |
| Distant | 12% |
| All stages combined | 77% |