What are 3T3-L1 cells?
3T3-L1 is a fibroblast that was isolated from the embryo of a mouse. This cell line can be used to study the basic cellular mechanisms associated with diabetes, obesity, and related disorders.
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How can you tell the difference between 3T3 and L1 cells?
Differentiation of 3T3-L1 cells into adipocyte-like cells
To initiate differentiation, remove DMEM and add 2–3 mL MDI induction medium per well (Day 0). On Day 3, remove MDI induction medium from the cells and replace with 2–3 mL insulin medium. On Day 6, remove insulin medium from the cells and add fresh DMEM.
What are 3T3-L1 preadipocytes?
3T3-L1 preadipocytes is the source of mouse embryos, having the morphology of fibroblast cells and the ability to differentiate into mature adipocytes under the stimulation of classic hormonal cocktails, including insulin, dexamethasone, and 3-isobutyl-1-methyl xanthine.
What are C3H10T1 2 cells?
The C3H10T1/2 cell line was established in 1973 from 14- to 17-day-old C3H mouse embryos (15). These cells display fibroblastic morphology in cell culture and are functionally similar to mesenchymal stem cells.
What are RAW 264.7 cells?
The RAW 264.7 cells are monocyte/macrophage-like cells, originating from Abelson leukemia virus transformed cell line derived from BALB/c mice. These cells are being described as an appropriate model of macrophages. They are capable of performing pinocytosis and phagocytosis.
What is a Preadipocyte?
Preadipocytes are precursor cells that develop into adipocytes when fully differentiated. Adipocytes perform essential functions of energy metabolism and are characterized by the accumulation of intracellular triglycerides. Preadipocyte Cells are isolated from subcutaneous or visceral fat.
What is hepg2 cell line?
Hep-G2 is a human liver cancer cell line that was derived from the liver tissue of a 15 year old Caucasian male with a hepatocellular carcinoma.
What is L929 cell line?
L929/A cells can be used in the development of novel anti-cancer treatments. Resistance can be circumvented by modulating agents such as verapamil and quinine. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.
What are c12 cells?
The c12 (B15ECiii2) cell line expresses reduced levels of aryl hydrocarbon receptor (AHR) mRNA and protein, due to an unknown factor required for AHR expression. This cell line is a useful tool to study the molecular mechanism of these processes.
Where is fibroblast found?
The fibroblast is a specific type of connective tissue cell that’s in our skin and in our tendons.
Are RAW 264.7 cells immortal?
Raw264. 7 is a cancer cell line and thus they are immortal. They proliferate very well and very fast, easy to crioprotect and also easy to thaw from liquid nitrogen.
Are RAW 264.7 cells adherent?
RAW 264.7 (ATCC TIB-71) is designated as an adherent line and is handled like a monolayer, however the culture can have mixed adherent and suspension populations. In the initial stages of culturing ATCC TIB-71, the cells attach and appear cuboidal-like with thin extensions.
Why is Adipogenesis important?
However, adipogenesis is a complex physiological process, necessary to sequester lipids effectively to avoid lipotoxicity in other tissues, like the liver, heart, muscle, essential for maintaining metabolic homeostasis and has a crucial role as a component of the innate immune system, far beyond than only being an …
How do you increase Adipogenesis?
Niacin. Niacin is the most effective for increasing HDL levels and decreases total cholesterol, LDL, triglyceride synthesis, and fatty acid re-esterification. It stimulates adipogenesis throughout increasing PPARγ, FABP4, adiponectin, and leptin expression in 3T3-L1 cells.
What media is used for HepG2?
Medium: The Hep G2 [HEPG2] (HB-8065) cells require the Eagle’s Minimum Essential Medium (EMEM) (ATCC 30-2003) with 10% Fetal Bovine Serum (FBS) (ATCC 30-2020) that has not been heat inactivated.
Are HepG2 cells tumorigenic?
But they differ in the number of chromosomes per cell as HepG2 cells contain an average of 55 (50–56) chromosomes per cell whereas Hep3B cells, 60. In addition, HepG2 is hepatitis B virus negative and non-tumorigenic, but Hep3B is hepatitis B virus positive and tumorigenic (Knowles et al.
What is C2C12 cell line?
C2C12 is a myoblast cell line that is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.
What are MG 63 cells?
MG-63, a cell that has fibroblast morphology isolated from the bone of a White, 14-year-old male patient with osteosarcoma. This cell line was deposited by A Billiau. Powered by Bioz See more details on Bioz. Homo sapiens, human.
What are 3T3 cells used for?
NIH/3T3 is a fibroblast cell line that was isolated from a mouse NIH/Swiss embryo. This cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies.
What is L6 cell line?
L6 cells fuse in culture to form multinucleated myotubes and striated fibers. The extent of cell fusion declines with passage and the cells should be frozen at low passage and periodically recloned with selection for fusion competent cells. Tested and found negative for ectromelia virus (mousepox).
Why are fibroblasts used in cell culture?
1. Fibroblasts are easy to grow in culture. As mentioned, the main role of fibroblasts is the production of ECM, which provides support for connective tissues and serves to maintain structural integrity. Fibroblasts effectively create their own environment, and as such, they are simple and easy to grow in culture.
Why are fibroblast cells used in research?
They can be easily isolated from different body sites and are easily cultured in vitro. Although biopsies are considered an invasive procedure, patient-derived skin fibroblasts have been reported as reliable sources for medical research purposes, with afford advantages over transformed cell lines.
What are RAW 264.7 cells used for?
How do you immortalize primary cells?
To immortalize your primary cells, you can use either hTERT or SV40 T antigens for cells that can be cultured for over 10 passages. It is recommended that SV40 T antigens be used for difficult–to–immortalize primary cells such as epithelial cells.
What causes Adipogenesis?
Overeating (calorie-overload), genetic predisposition, early development and physical inactivity are main factors contributing to energy imbalance (input > output) of an individual. In the presence of excess energy, already mature adipocytes start to enlarge, undergo cellular hypertrophy and store more fat 10.